FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16172393
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG288830.17859447269182738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC87350.067.48869
TTCCGAC89950.065.537648
TTTCCGA102550.057.5317347
GTTTCCG89100.055.60586
CGTTTCC94400.050.8745655
GCTCCGT70200.050.426051
CCGTTTC96850.048.9107634
CTCCGTT77350.048.098832
GTTCCGT23150.048.0286751
TCCGTTT97700.047.613113
CCCCGTT37950.036.434622
GGTGTGC122350.035.563546
GTGCTCT157200.035.536649
GTCCCGT21200.034.067751
CGGTGTG41850.033.1443065
CGACCTG94700.032.4543710-11
GCCCCGT31200.032.2862051
CCCGTTA20500.032.218063
CCGACCT95800.032.15609710-11
CCCGTTT50300.031.8346733
Produced by FastQC (version 0.11.5)