FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7964622
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT808471.0150764217058887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343230.43094323873750695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332810.4178603830790714No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157360.19757372038497245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92600.083.5709461
GTATCAA294500.072.954391
ATCAACG370700.057.717053
TCAACGC372550.057.4304354
CAACGCA375550.056.9223675
AACGCAG389600.054.9494676
ACGCAGA419300.050.968657
CGCAGAG423100.050.5249378
TATCAAC425000.050.455132
GCAGAGT455350.046.9726459
GCAGCGC12200.043.8664638
TCTGTCG13950.036.6584558
GTCTCGC14100.033.3270571
AGAGTAC487300.032.7884710-11
GCTCTAG17400.030.424941
CAGAGTA489350.029.81373410-11
AGTACTT500600.028.42508512-13
CTCGCTC16550.026.9556333
TGCAGCG20050.026.691817
CGCTCTG16450.026.3954815
Produced by FastQC (version 0.11.5)