FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8280650
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2111772.550246659380604No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1682012.0312535851654157No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1017671.2289735709153267No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG641830.775096157910309No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC483450.5838309794520962No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG474340.5728294276415499No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT435220.525586759493518No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC352620.4258361360521215No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA281850.34037183071377247No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC270810.32703954399715No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC250270.30223472795010053No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG237530.2868494623006648No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG180310.2177486066915037No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC174180.21034580618671242No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA153740.18566175360629902No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA139230.1681389745974048No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG139140.16803028747743234No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA136970.16540972025142955No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG125910.15205328084147984No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA117890.14236805081726678No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC117370.14174008079075917No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC117210.14154685924414145No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC116100.14020638476448105No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG113390.13693369481864345No Hit
CCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCG108020.13044869666028633No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC98710.11920561791646792No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC93060.11238248205152977No Hit
CCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGA90820.10967738039888172No Hit
CCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAG90450.10923055557232825No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG88850.10729834010615108No Hit
TACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGG87130.10522120848001063No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT83280.10057181501452181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT19550.068.273555
TGCGACA20050.066.097164
CTGCGAC22450.059.030743
GCTGCGA25400.052.5653272
GCTCCCG344800.050.0150268
ACCCGAC442400.049.904693
AACCCGA443650.049.694032
CGACTCC445500.049.450816
GGGCTCC349400.049.3213236
CCCGACT447300.049.3052064
CCGACTC446750.049.248955
GACTCCC450000.049.0592967
GGCTCCC352650.048.8747567
CGGGGCT353400.048.7096064
ACTCCCT456800.048.3081938
CCGGGGC359000.048.1611983
CCCGGGG358450.048.091182
GGGGCTC365350.047.15545
CTCCCTT480650.045.9001279
CTCCCGC381600.045.115999
Produced by FastQC (version 0.11.5)