FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10884868
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC39800.046.937859
TTCCGAC40550.046.186638
TTTCCGA52200.036.060867
ATAGCGC75850.035.19738894-95
CCGATCG80200.034.5549386-87
GTTTCCG46950.034.538756
CCGTTTC52700.034.2832834
GTCCCGT14150.033.854081
GCCCCGT23150.033.3950921
GATCGGC84600.033.1489788-89
TCACCCC151350.033.0506559
ACCCGAT84050.032.9923784-85
CGATCGG81450.032.80008786-87
CTCCGTT44300.032.5473672
GTTCACC153800.032.4937867
CGTTTCC51100.031.9229935
TCCGTTT55600.031.7393633
CCGTTAT12050.031.482634
CGGCATA85400.031.36951890-91
CATAGCG89650.031.34253794-95
Produced by FastQC (version 0.11.5)