FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9501156
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG945860.995520966080338No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG468330.4929189669130788No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA460200.4843621134101998No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG410210.43174746315079976No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG318840.33558021781770553No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA263850.27770305002885964No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG213330.22453057291133838No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG207410.21829975215647443No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA195650.2059223109272177No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG181500.19102938631888583No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA181260.19077678547747243No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA165950.1746629568023091No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA153660.1617276887149311No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA152160.16014893345609732No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG142360.1498343990983834No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA110860.11668053866287428No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT102290.10766058361740403No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA100340.10560820178092012No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA97930.10307166833172722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTTA87950.062.0278972
GCTAGTA60350.061.4938855
GATAGTA92000.061.205255
GGTGCTA60900.057.2743032
CGCGATA2500.057.02493
GTGCTAG61850.056.855873
TGCTAGT66600.054.7299464
GTGATAG84000.054.4791533
ATAGTAG120000.053.2816856
GGTGATA87850.050.847212
TGATAGT94500.050.1380274
GTAGCAA889650.049.760359
TAGTAGC884950.049.5712627
AGTAGCA893050.049.531228
GTGTTAG113250.048.9684073
AATACCG23800.047.3024068
CGTAGTA28400.046.6814165
TTCGACG4250.044.7221151
TTAGTAG144450.043.868466
TGGTGTT117650.043.3370251
Produced by FastQC (version 0.11.5)