FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23939526
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC646830.27019331961710524No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC342810.14319832397684065No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG293350.12253793161986581No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC260980.1090163606413928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA84450.042.2454534
GCGACAT87150.040.8782925
CTGCGAC92100.039.203223
GCTGCGA100250.035.971072
CGACATC102100.035.1693276
CTAGGCG238950.031.0276857
TAGGCGG242100.030.6240438
GGTGTGC234450.029.7392027
CCGCTCG20950.029.6989257
TCGACCG20550.029.5914273
GCTCGCG21700.028.0159029
GGCTGCG131700.027.7431951
GCGCGGT101500.027.6092076
GCTAGGC270950.027.5744956
GTGTGCT362300.026.4942958
GGCTAGG287950.026.3607965
TGGCTAG293900.026.2821334
CGCGTCG11950.026.03397612-13
TGTGCTC311050.025.408419
CGACCGC26050.025.3481774
Produced by FastQC (version 0.11.5)