FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23939526
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG248820.10393689499115395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC351000.054.688256
GTGCTCT472500.052.7905439
CGGTGTG118450.051.2135055
TGTGCTC492500.050.801098
TCCGACC84650.047.2499169
TTCCGAC86050.046.5915878
CCGGTGT72850.046.0448344
GTGTGCT561950.045.156987
TTTCCGA105100.038.5536047
GTTCCGT30550.037.1716231
GTTTCCG93350.036.383466
CGGGGAT195100.036.326838
GCGGTGT44550.036.2607654
TCGGGGA197450.036.014997
CCGTTTC111250.035.4900634
CCCCGTT60400.034.4554862
CGGGTGT75500.034.2340054
CCGATCT373600.034.13205716-17
TCCGTTT111950.033.7847063
GGGTGTG300700.033.4438365
Produced by FastQC (version 0.11.5)