FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16419185
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA338710.20628916721505972No Hit
TCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCA325080.19798790256641852No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAA222400.13545130285090276No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC208020.12669325548131652No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT184140.11214929364642642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT42600.049.061415
TGCGACA43250.048.3295174
CTGCGAC48950.042.607193
GCTGCGA54250.038.8852272
CGACATC53750.038.8814136
ACGGACC68000.034.5813674
CGGACCA70050.033.4977575
GGCTGCG64700.032.8264961
CACGGAC74400.031.9277343
ACACGGA85600.028.529282
AACACGG88050.028.0065291
TCGCACG63100.025.48297142-43
CGAGTCG65100.024.69816630-31
CGCGAGT66000.024.2890728-29
CGGTCGA16250.024.26087468-69
TGCGCGA66700.024.10552226-27
TAGTGCG155250.023.4036967
GCGCGGT33500.023.1105696
TTGATCG46250.022.6446722-23
AGTGCGC160050.022.6424548
Produced by FastQC (version 0.11.5)