FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17063352
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT3521792.0639496858530495No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT3225251.8901620267811388No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG2256401.3223662033110493No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1332160.7807141293223043No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1216860.7131424118778069No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG649000.3803473080787409No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC639020.3744985158836318No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT551220.323043209798403No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC443080.259667619820537No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC412300.2416289601245992No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC399710.2342505739786649No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC396590.23242209385354062No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC386340.22641506780144957No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC385050.2256590615958693No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG353120.206946442879453No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA345630.20255691847651033No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA331280.19414708200358288No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA284770.16688983501014337No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA277470.16261166035841024No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG262290.1537154012880939No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT259070.15182831603075408No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC253810.14874568607621763No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA235370.13793889969567527No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC233680.13694847296123294No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC228380.13384240095381025No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG227980.13360798042494818No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC226420.13269374036238601No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT223230.13082423664471085No Hit
TACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCT221740.12995102017469953No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT215110.1260654999088104No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC214100.12547358807343365No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGT209600.12283635712373511No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT200720.11763222138299673No Hit
TGGGGGGGATTCTTCTCTAATCTTTCAGAAACTTTGTCTGCGAACACTCT188460.11044723217337367No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT174240.10211358237232637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG448050.071.5418268-69
TGCGACA56100.068.8715064
GCGACAT56950.067.994515
CTGCGAC60000.064.394863
GCTGCGA63600.060.9515232
AACCCGA498300.058.9153632
ACCCGAC499250.058.864543
CCCGACT506800.058.0469784
CCGACTC508600.057.782395
CGACTCC514000.057.1443946
GACTCCC522600.056.1957937
ACTCCCT539150.054.4229558
TCTCCGC230150.052.69287568-69
GGCTGCG75450.052.3704341
TTGAATA318000.051.08694576-77
CTCCCTT575600.051.0363359
TGAATAT335200.048.94797576-77
GAATATT348100.046.59042478-79
GAACCCG647700.045.8240931
CGTAGCG11350.044.2839676-77
Produced by FastQC (version 0.11.5)