FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14491866
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG362910.25042323742159917No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG192170.13260542155164834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC92950.071.037629
TTCCGAC94050.070.35838
TTTCCGA104850.063.3829277
GTTTCCG93550.062.558166
GCTCCGT67500.057.442761
CGTTTCC100100.056.0936785
CCGTTTC99650.055.3586964
CTCCGTT77300.054.1552852
TCCGTTT101300.052.9118653
GTTCCGT22650.050.9784931
TGTCGGG27200.040.8674435
CTGTCGG28900.038.307964
CGGGGAT131600.037.396158
TCGGGGA134200.036.9194157
CCCCGTT36700.036.899732
CGACCTG96250.035.6582610-11
CCCGTTA18650.035.4151763
GTCCCGT19600.034.4255751
CCGACCT100350.034.177710-11
CCCCCGT29950.034.110691
Produced by FastQC (version 0.11.5)