FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14857253
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG166000.11172994092514948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC42050.042.326169
TTCCGAC47350.037.786938
CGGGGAT143850.036.522298
TGTCGGG34850.035.272385
TCGGGGA149750.035.0199627
GTTCCGT23700.033.0449831
CCCGTTA19600.032.4488753
CCCCGTT38900.032.4483222
CCCCCGT29850.031.6430761
CCGTTAA17850.031.1098984
GTCCCGT23100.030.8211771
CTGTCGG37950.030.5160964
CCGATCG64050.029.0027986-87
CCGTTAT16050.028.980244
CTACCGA9900.028.7664321
GCCGTTA9800.028.0900733
ATAGCGC62150.028.04908494-95
CCCGTTT49900.027.7736683
CCGTTTC68250.027.6082444
GATCGGC68300.027.54539588-89
Produced by FastQC (version 0.11.5)