FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13508527
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATG1231870.9119203004146935TruSeq Adapter, Index 1 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTAT166130.1229815804491489TruSeq Adapter, Index 1 (97% over 37bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTC147990.10955302528543637TruSeq Adapter, Index 1 (97% over 37bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCT138740.10270549853437017TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)