FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19223695
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATG2369781.2327390753962753TruSeq Adapter, Index 9 (97% over 36bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTAT377160.19619537242970198TruSeq Adapter, Index 9 (97% over 36bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTC337930.17578826547133627TruSeq Adapter, Index 9 (97% over 36bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTA288620.15013762962843513TruSeq Adapter, Index 9 (97% over 36bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTC277190.14419184241114938TruSeq Adapter, Index 9 (97% over 36bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCT264630.13765823895978374TruSeq Adapter, Index 9 (97% over 36bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTAT250520.1303183389041493TruSeq Adapter, Index 9 (97% over 36bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTAT210280.10938583867461484TruSeq Adapter, Index 9 (97% over 36bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTA203180.10569248003570594TruSeq Adapter, Index 9 (97% over 36bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCT194850.10135928602695787TruSeq Adapter, Index 9 (97% over 36bp)
ACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCG194620.10123964201471153TruSeq Adapter, Index 9 (97% over 36bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTA194420.10113560374319297TruSeq Adapter, Index 9 (97% over 36bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTA192620.10019925929952592TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)