FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19223695
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2158591.1228798625862508Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC339960.17684425392724967Illumina Single End PCR Primer 1 (100% over 49bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG305890.15912133437406284Illumina Single End PCR Primer 1 (100% over 45bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG264250.1374605662438985Illumina Single End PCR Primer 1 (100% over 48bp)
GGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG253880.1320661818656611Illumina Single End PCR Primer 1 (100% over 45bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG240260.1249811755752471Illumina Single End PCR Primer 1 (100% over 44bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC231640.12049712607279714Illumina Single End PCR Primer 1 (100% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC194480.10116681522464854Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)