FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10911037
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC2115000.048.9956786
CGGTGTG709800.048.3245055
CCGGTGT311400.048.0372664
GGGTGTG1040550.047.20635
GCGGTGT176800.046.9002724
CGGGTGT241700.046.7123764
GTGCTCT4034050.044.7941329
TGTGCTC4033850.044.7056738
GTGTGCT4095050.044.431987
GGGGTGT365750.044.3418854
ACGGTGT160350.043.0584954
GTCCGGT29900.042.2836272
TCCGGTG95150.042.1097373
ACGCGGT19950.041.930642
CCCGGTG117050.041.3371123
TCGGTGT107750.040.6166954
GCCGGTG88000.040.5081443
CCGGGTG138400.040.249023
ACCGGTG78050.039.582593
AGGGTGT245450.038.9324044
Produced by FastQC (version 0.11.5)