FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13811186
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC369650.2676453709333869No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC212290.153708740147298No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT196740.14244975051382264No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC192740.13955354739267142No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT188160.13623739481895328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA57950.065.412524
GCGACAT58850.064.4879765
CTGCGAC63000.060.17483
CGACATC69250.054.6647766
GCTGCGA70350.054.0274432
GGCTGCG92650.041.4871521
ACTCGGG123900.032.2887571
CTCGGGA133700.030.1337342
TCGGGAG138600.029.1345313
TTGATCG66500.028.74847422-23
ATTCGGC66450.028.62751238-39
TGATTCG69550.027.421336-37
TTCGGCT70300.027.23040640-41
GACATCT140200.027.136487
ACATCTG142200.026.7887978
GGTGTGC109350.026.7131637
TCGGCTG72850.025.9838340-41
TCGCCAG74700.025.7846526-27
TGATCGC73800.025.77639824-25
CATCTGT157700.024.2461419
Produced by FastQC (version 0.11.5)