FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15040518
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC313640.2085300519569871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA44650.067.5573044
GCGACAT45500.066.185125
CTGCGAC48900.062.272763
CGACATC53300.056.587296
GCTGCGA53900.056.412242
GGCTGCG65500.046.425381
GGTGTGC140050.032.7955067
TTGATCG49100.030.90745222-23
TGATCGC50750.029.99642224-25
TGTGCTC184750.029.0773539
ATTCGGC51950.029.02894838-39
TGATTCG52550.028.83423436-37
TTCGGCT52250.028.81840340-41
GTGTGCT208000.028.3847148
GATCGCC54200.028.04323624-25
TCGGCTG55550.027.2346840-41
TCGCCAG55700.027.07610526-27
GATTCGG57250.026.38303438-39
GACATCT124300.024.7995917
ATCGCCA64400.023.52894826-27
Produced by FastQC (version 0.11.5)