FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17341399
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC272040.15687315654290637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT40500.067.201845
TGCGACA40200.066.9980854
CTGCGAC43750.061.6757553
CGACATC45250.059.937396
GCTGCGA48800.055.4918632
GGCTGCG61900.044.441481
ATTCGGC43500.031.2831238-39
TTGATCG43400.031.24535422-23
TGATCGC45750.029.84808224-25
GATCGCC46650.029.52677724-25
GGTGTGC125300.029.3406817
TCGCCAG46700.029.08963426-27
TTCGGCT46900.029.06803540-41
TGATTCG47200.028.932636-37
TCGGCTG48750.028.01366240-41
GATTCGG52000.026.12386138-39
CGGCTGA55800.024.47465342-43
ATCGCCA56450.024.06529626-27
GTGTGCT193950.023.68228
CGCCAGG57950.023.44237728-29
Produced by FastQC (version 0.11.5)