FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15467900
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG154930.10016227154300197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC140750.048.536666
GTGTGCT186550.047.2173847
CGGTGTG50100.046.8526655
GTGCTCT190250.045.799659
TGTGCTC206650.041.9580158
CCGGTGT34950.041.8784644
CGGGGAT121250.038.870268
TCGGGGA123550.038.3773777
TCCGACC34700.037.789159
TGTCGGG29750.035.9369055
TTCCGAC37150.035.169118
CCGATCT126850.034.48567216-17
TCCGATC122100.034.3101416-17
CCCCGTT36000.033.1383972
CCCCCGT29150.032.936811
TTCCGAT134900.032.6754114-15
ATCGGGG86750.032.5294236
CCCCGAA37850.032.3976942
CCCGTTA19000.031.5196533
CCGTTAA17650.030.9628074
Produced by FastQC (version 0.11.5)