FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19851442
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG222710.11218832364923415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC86350.050.7720269
TTCCGAC90150.048.5265058
TTTCCGA109750.040.2492567
GTTCCGT32000.036.9270171
GTTTCCG104400.036.5398866
GTTCACC340500.035.72847
TCACCCC341150.035.5492449
CCGTTTC116950.034.9312824
TTCACCC356150.034.171838
TCCGTTT118700.034.1364863
CTCCGTT92150.033.6725042
ATAGCGC183000.033.5864194-95
GCTCCGT89050.032.8810771
CGTTTCC115550.032.685475
CCGATCG197400.032.5486186-87
CGGGGAT90800.031.5620298
ACCCGAT208000.031.08891784-85
GATCGGC207950.030.91562888-89
CGATCGG199800.030.77859986-87
CATAGCG210300.030.60415694-95
Produced by FastQC (version 0.11.5)