FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20943006
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN647330.3090912546174126No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297600.1420999449649205No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGT45850.032.4720541
TAGTAGC203000.032.112257
GTAGCAA205800.031.8900329
CGGGGAT127700.030.8862848
AGTCGGT146800.028.77749686-87
AGTAGCA229200.028.6531188
TCGGGGA138800.028.6173537
TCAGTCG147450.028.55496884-85
GTCCCGT31200.028.5096421
CGAGAAC149750.028.48189524-25
ACGAGAA155450.028.4452524-25
CCCCCGT47200.028.1168691
AAGGCCG158300.027.96301336-37
AAACGAG160350.027.67893222-23
TGTCGGG33750.027.3114535
CGAAGTG155250.027.27294740-41
CCGTTAT22350.027.2107734
CAGTCGG150800.027.18048384-85
TCGGTCC152500.027.12572988-89
CAAACGA156800.027.11497120-21
Produced by FastQC (version 0.11.5)