FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9322933
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT222650.23881969333041436No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC183070.1963652425690499No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG155410.16669646773177496No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC116170.12460670906891641No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG97300.10436629760183838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA25550.052.8110124
GCGACAT25850.052.74955
ACCCGAC51450.045.155313
CTGCGAC31450.043.3557433
CCCGACT55100.042.596354
CCGACTC55450.042.070435
CGACTCC56750.041.10676
AACCCGA58900.040.1078342
GCTGCGA36400.037.6079142
GAACCCG78350.031.028241
GACTCCC77050.030.520227
CTAGGCG68000.030.110887
GTATTCG19400.029.6728231
TAGGCGG70100.029.0039778
TCGATCG42650.028.00882116-17
ACTCCCT84300.027.8939118
ATTCGTA21200.027.3406813
GGCTGCG53900.026.8324341
TTCGATC44550.026.59990314-15
CGGAACG44650.026.54353556-57
Produced by FastQC (version 0.11.5)