FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19470261
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG759290.38997422787501407No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG283840.14578130205855996No Hit
CCTCTCTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG197040.10120049238168918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG61550.051.3220255
CGGGGAT406250.046.8644378
TCGGGGA407100.046.825057
GTCGGGG78300.044.1044926
CTGTCGG69550.043.4380344
TCCGACC56950.042.1210259
ATCGGGG303050.039.938836
TCTGTCG75900.039.8659673
TCTATCG227400.037.8087843
TATCGGG252200.037.594625
TTCCGAC64500.037.4858328
CTATCGG240950.037.0827644
CATCGGG45550.036.4997525
CCCCCGT45900.035.290111
GGGGATG550700.034.8040479
CGTCCGT12450.034.720291
CCCCGTT55000.033.4234922
TCGATCG7250.032.7594533
CCCCCGA48950.032.4119451
TTTCGGG54350.031.9883425
Produced by FastQC (version 0.11.5)