FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004913864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17994994
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT9375155.209865588174133No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2497671.3879804572316057No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1379630.7666743317613777No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1292780.7184109091672939No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC1280090.7113589479385212No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG880480.48929163299526524No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC835610.46435692059691713No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG671210.3729981793825549No Hit
CAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAA570680.3171326425560353No Hit
CTGAAAACTTCCGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCAT549830.30554608687282697No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC512970.2850626124132078No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG511620.284312403771849No Hit
ACCCGCCGTGTGTCTCCCGTGATAACATTCTCCGGTATTCGCAGTTTGCA469450.2608781086562185No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT451790.2510642682070358No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA415560.2309308911133841No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT395170.21959996207834243No Hit
GTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTC308950.17168663685022623No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA291250.16185056799685513No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC279900.155543258308394No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG279180.15514314703300264No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC265500.14754103280056663No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC256160.14235070042257308No Hit
CAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGG253600.1409280825545149No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC252710.14043350056132278No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA238500.13253685997339038No Hit
AGCCCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGA235780.13102532848857854No Hit
GATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTC223860.12440126404043257No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA222930.12388445364305206No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC219810.12215063811635615No Hit
CCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC219280.12185611176085971No Hit
ACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTC211900.1177549711880982No Hit
ATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATT206940.11499864906873544No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG206090.11452629547973175No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC201110.1117588591582748No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG200160.11123093455880008TruSeq Adapter, Index 6 (100% over 49bp)
AGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGT196440.10916369296927803No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG192350.10689083864101318No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT189150.10511256630594043No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA188470.10473468343473746No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC183330.10187833349652688No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT182180.1012392668761101No Hit
AGCCCCCCTCAGTTCTCCAGCGCCCACGGCAGATAGGGACCGAACTGTCT180910.10053351504312812No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)