FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17994994
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1806711.0040070032810235No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1083620.6021785836661018No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC954250.530286367419739No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA895160.4974494573324114No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG827100.45962782760583304No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG681290.3785997372380341No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG624950.34729102993865957No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC581610.3232065540005181No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG504490.28035019072526507No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC458240.25464859838241677No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA458240.25464859838241677No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA419960.23337601557410914No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG417400.23195339770605092No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG401000.2228397519888031No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA389460.216426857380447No Hit
GGTGGTTCCGCATGGAAGGGCCATCGCTCAACGGATAAAAGCTACCCCGG378810.21050854476528305No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA354560.1970325747260599No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA353930.19668247736009248No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG313750.17435404535283536No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG298790.16604062218637028No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA270000.15004172827176268No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC265920.14777443104454496No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG250640.1392831806445726No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA240500.13364828018281083No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA227840.12661299025717931No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG225930.12555158395718277No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA220950.1227841476357258No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC217190.12069467764201532No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA210190.11680470690904371No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC207000.11503199167501807No Hit
GGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCACGAAGGTTGGCTAAT206990.11502643457397096No Hit
TGATTCTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC205770.1143484682462245No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201310.11187000117921685No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA201030.11171440234989798No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA190350.1057794184315927No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA188790.10491251066824472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTA150550.072.121795
TGCTAGT105800.071.25084
GTGCTAG102400.071.200973
GGTGTTA185100.070.068052
TTCGACG8350.063.222871
TGATAGT157300.059.533744
TTAGTAG238100.056.6813856
CAGTACG366900.056.6035168
TCGCGTG4300.056.408623
TGATCGT50000.056.120735
GGTGCTA132050.055.789742
GTGATAG161700.055.7663353
TTCAGTA295100.055.269556
TCGACGT10900.054.977552
TCAGTAC385950.053.8866847
CGTAGTA37500.053.3939675
TGGTGCT144500.051.8382571
GTACACC77250.049.9064677
GCTCCGT48450.049.7681961
AGTAGCA1792400.049.4094858
Produced by FastQC (version 0.11.5)