FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8818641
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT921061.0444466443298916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406970.46148834043703557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372900.4228542697225116No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG187670.21281056797753756No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC171680.19467852246168088No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152760.17322396954360655No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC141480.16043288302585398No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG120940.1371413123632088No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA119620.13564448308985477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99340.11264774243559751No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG97750.11084474353814833No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT97340.11037981929415203No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG96300.10920049926060037No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC94640.10731812305320058No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA90350.10245342791480001No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG88950.10086588171578818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102550.091.834331
GTATCAA338850.070.4483951
GCAGCGC15300.058.7199638
ATCAACG423550.056.147333
CAACGCA428100.055.9399155
TCAACGC429650.055.6272664
TGCAGCG16600.054.4807557
AACGCAG448800.053.4024856
GCTCTAG14550.052.369841
ACGCAGA485250.049.3278437
CGCAGAG487300.049.143918
TATCAAC508150.047.0357252
GCAGAGT515500.046.4439939
TGGTCCT38800.045.5660865
GTCGTGC10000.035.1225661
TGGTATC30500.034.351612
CATGCAG27400.033.2397585
GTGGTAT34000.031.8659381
CTAGCCT75350.031.363484
AGAGTAC570000.031.20498310-11
Produced by FastQC (version 0.11.5)