FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17951224
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358950.19995850979298127No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA303500.16906925121094807No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG277340.15449642876719716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAC12950.044.4248546
CGACTGA139350.035.9282049
CACCGTC105000.035.229339
GTACCGA16950.033.685445
TCTGTCG42700.033.539733
CCACCGT109650.033.518538
CCGACTG151850.033.189848
TCCGACC61750.032.184999
ACCGACT156550.031.9805367
ATAGCGC102900.031.86154494-95
CCGTTTA47250.031.6198434
CTGTCGG47350.031.1511154
CCGATCG114400.030.8128286-87
TCACCCC206500.030.7149549
ACGGTTA14100.029.3583764
GCCACCG127400.028.9229587
GACCGAC121050.028.8690836
TGTCGGG51450.028.6686345
ACCCGAT126700.027.76504184-85
CGGCATA120000.027.49798490-91
Produced by FastQC (version 0.11.5)