FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19243519
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC326040.16942847095689723No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC305250.15862483363879548No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC227470.11820603082003868No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG197500.10263195624459331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA62100.051.4455644
GCGACAT69500.046.5829965
CTGCGAC77400.041.7057383
GCTGCGA79550.040.818422
CGACATC113950.028.2027116
GGCTGCG121250.027.3553941
GGTGTGC135600.024.9601067
GCGCGGT58150.022.3674966
GTGTGCT198100.021.973618
CTCACGT99250.021.235829
CGGTCTA50100.020.750236
CGACGGT50150.020.6354243
TGATCGC78650.020.49137724-25
TTGATCG79700.020.39957222-23
GACATCT163050.020.117497
CTAGGCG175850.019.7869197
TAGGCGG179850.019.2412668
GCTAGGC184250.018.8075376
TGTGCTC164200.018.646759
GGCTAGG195000.018.5745285
Produced by FastQC (version 0.11.5)