FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10526483
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG113270.10760479069789977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC31450.041.3548058
TCCGACC31500.041.1476679
GTAGCAA136150.038.359089
TAGTAGC140500.036.922447
CGCGAAC3000.036.3861925
AGTAGCA152250.034.450948
CCCGTTA11650.032.99673
TTTCCGA39800.032.5556347
CCCCGTT25100.032.5199362
GCCCCGT18500.032.3164441
TCAGTCG107900.031.71290884-85
ATAGCGC33200.031.61092694-95
AGTCGGT109350.030.93924786-87
TCGGTCC107850.030.92661788-89
CGAGAAC110550.030.86501724-25
AAACGAG114150.030.83141522-23
CAAACGA111000.030.81124520-21
CAGTCGG109050.030.74654884-85
CCGTTTC45250.030.6262844
AAGGCCG114900.030.51058436-37
Produced by FastQC (version 0.11.5)