FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8800606
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT413150.4694563078951609No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG206280.23439294975823255No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC157050.17845362012570498No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT109500.12442324994437882No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG99600.11317402460694183No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT94400.10726534059131837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT29300.058.2113765
TGCGACA29050.057.894614
ACCCGAC85050.048.5421523
CTGCGAC34850.048.5311743
AACCCGA88950.046.649482
CCGACTC89650.045.4692735
GCTGCGA37950.044.337372
CCCGACT93400.043.7962844
CGACTCC95550.042.611686
GACTCCC103600.039.342927
GAACCCG120450.035.3099441
ACTCCCT118050.034.7273378
CGGGGCT66750.034.0218284
GCTCCCG68050.033.5073288
CCGGGGC71850.032.333723
GGCTGCG55000.031.658091
CCCGGGG74200.031.2603912
CTCCCTT142600.028.8819359
CTCCCGC78150.028.8729619
CGACATC60100.028.3000456
Produced by FastQC (version 0.11.5)