FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8800606
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326650.3711676218660397No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG143580.16314785595446496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGC168500.043.2352337
GTAGCAA176550.041.4952059
AGTAGCA181550.040.5503128
TCCGACC37100.039.1604089
TTCCGAC39300.036.9579248
TCACCCC112650.034.476399
TTTCCGA43200.034.1669277
GTAGTAG145000.032.5674976
ATAGCGC66850.032.0370694-95
ACGAGAA157250.032.02652724-25
AAACGAG157900.032.01899722-23
AGTCGGT153200.031.96279386-87
TCAGTCG154350.031.9608284-85
CGAGAAC155300.031.26667624-25
TCGGTCC157700.031.10842588-89
CAAACGA156800.031.00534820-21
CAGTCGG154800.030.9315284-85
GGTAGTA116850.030.908145
CGAAGTG158350.030.5541340-41
AAGGCCG166550.030.2753236-37
Produced by FastQC (version 0.11.5)