FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7159370
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT421510.5887529209972385No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG280770.3921713782078591No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG108730.15187090484218582No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG77660.10847323158322589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAC81900.051.0324133
CCGACTC85250.048.6927575
AACCCGA88200.047.7641872
CGACTCC87450.047.517786
CCCGACT88700.046.798854
GCGACAT12250.046.5256545
TGCGACA13100.042.4191474
GACTCCC98850.042.1335267
ACTCCCT109250.038.252528
GCTCCCG67850.037.235798
CGGGGCT69350.036.4344754
GAACCCG118000.036.253821
CCCGGGG72300.035.539092
CCGGGGC72700.035.0169033
CTGCGAC16800.033.9249573
CTCCCTT129400.032.2591869
CTCCCGC78700.032.162619
CCCCGGG81500.031.7274071
GCTGCGA18850.030.739432
GGGGCTC86300.029.9388735
Produced by FastQC (version 0.11.5)