FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7159370
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272510.3806340502027413No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG163070.22777143798965555No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG82610.11538724776062699No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA79470.11100138699354832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGC167950.043.584517
GTAGCAA175950.041.9787459
AGTAGCA180350.040.8509528
GTAGTAG138350.033.0988966
TCAGTCG156550.032.70128384-85
AGTCGGT155900.032.68456386-87
TCGGTCC157700.032.2196588-89
AAACGAG160200.032.15281322-23
ACGAGAA161750.031.94680424-25
AAGGCCG161650.031.92271636-37
CCCGTTA7350.031.678233
CGAGAAC157700.031.17105324-25
CAAACGA158400.030.95855920-21
CAGTCGG157150.030.92871384-85
GGCCGAA167300.030.85893638-39
CGAAGTG160650.030.51103440-41
GTCGGTC158850.030.43257386-87
GCCGAAG162750.030.2333438-39
TCAAACG170850.030.14813220-21
GGTAGTA113300.030.0288225
Produced by FastQC (version 0.11.5)