FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14745859
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT680890.461749973331496No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG378610.25675682915454434No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC251150.1703190027790175No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG170410.11556464767498455No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT163400.11081077067127795No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT149310.10125554570947681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA42700.055.459254
GCGACAT43900.054.0411835
ACCCGAC138950.045.1590273
CTGCGAC53450.044.7071883
AACCCGA144300.043.632912
GCTGCGA55500.043.132552
CCGACTC146750.042.8173265
CCCGACT151600.041.504214
CGACTCC158450.039.7316676
TCTCCGC94250.038.2539668-69
GACTCCC170700.036.880397
ACTCCCT188600.033.402868
GTTCTCG46250.033.07760668-69
GAACCCG198250.032.5341151
CGGGGCT113050.032.2385524
GCTCCCG119000.030.6946958
GGCTGCG80650.030.4966831
CCGGGGC122450.030.2200153
CCCGGGG128600.029.108522
CGCCCAT218350.028.7339668-69
Produced by FastQC (version 0.11.5)