FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8899184
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT949781.0672663920647105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427200.48004401302411553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390190.4384559303414785No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG193650.2176042207914793No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC173610.1950853022029885No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157780.17729715443573255No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC143460.1612057914523399No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG126150.1417545698571914No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA121540.13657431962301264No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103550.11635898302585945No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG101250.11377447640143186No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT100480.11290922853151479No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG96600.1085492782259587No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC96530.10847061932869351No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA92510.10395335122860704No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG89860.1009755501178535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105900.090.275431
GTATCAA339750.070.925271
GCAGCGC14300.062.8269658
TGCAGCG15650.057.4106147
ATCAACG426750.056.1283723
CAACGCA430200.055.8024645
TCAACGC434650.055.258544
AACGCAG453050.053.0352256
ACGCAGA487550.049.231877
CGCAGAG490150.049.0043838
TATCAAC515850.046.6898422
GCAGAGT519650.046.25689
GCTCTAG13350.045.481931
TGGTCCT39200.043.2767225
TGGTATC32400.039.1340262
GTGGTAT33900.038.807221
GTCGTGC9450.037.795441
CATGCAG26650.034.396885
AGAGTAC573500.031.0823310-11
CAGAGTA552750.030.41925610-11
Produced by FastQC (version 0.11.5)