FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5141254
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT316250.6151223028467374No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG178390.34697760507455966No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG75930.14768770420601668No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG56650.11018712555341556No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC54060.10514944408504229No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT52200.10153164967146149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAC58850.052.2295763
CCGACTC60150.050.944785
CGACTCC61600.049.7455946
AACCCGA62900.049.0424922
CCCGACT63150.048.8255274
GACTCCC69900.043.9721157
GCGACAT8900.043.23855
TGCGACA9100.041.7661254
GCTCCCG47050.040.2860878
ACTCCCT80400.038.228748
CGGGGCT50150.037.9882284
CCGGGGC52300.036.7884983
CCCGGGG53300.036.2949032
GAACCCG86200.036.1543661
CTCCCGC55000.034.290179
CTCCCTT92400.033.418219
CCACTTA41600.032.9061932
CTGCGAC11600.032.763533
CACTTAT42250.032.2711073
GGGGCTC59600.032.0446435
Produced by FastQC (version 0.11.5)