FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6836382
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG430770.6301139988959072No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA230590.33729829608702383No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG230120.33661079793376086No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG198130.2898170406510344No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG159780.23372011686883498No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG108850.15922164677164033No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA104200.15241980334042188No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG102280.14961130024624136No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG92980.1360076133838045No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA85850.12557812012260286No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA80490.11773771565134891No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA79800.1167284098518778No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG71720.10490929266386811No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA70490.10311009536915872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTA38100.049.445955
GATAGTA55450.049.1219445
TAGTAGC451650.048.7441567
GTAGCAA464300.047.9260189
AGTAGCA465500.047.738198
ATACCGG13300.047.119149
GGTGTTA58750.045.3104022
ATAGTAG69300.045.265286
AATACCG13850.044.8927048
GGTGCTA37900.044.821542
GTGCTAG39650.043.682293
GTGATAG53350.041.8930663
CGTAGTA15250.041.6965565
TGCTAGT45000.040.7042734
GTTAGTA70600.039.3874745
GTAGTAG360650.039.041696
CGGTAGT11950.038.518475
GTGTTAG71900.037.7505073
GGCGGTA22500.036.4854133
CCGCGTT2350.036.3452452
Produced by FastQC (version 0.11.5)