FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16189728
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC280090.17300475956112418No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC193870.11974876909605894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT42650.064.815965
TGCGACA43750.063.4091224
CTGCGAC49450.056.4885143
GCTGCGA56350.049.659392
CGACATC62400.044.5285536
GGCTGCG71650.039.853131
GGTGTGC149500.033.4544947
ACTCGGG94700.029.5508171
GTGTGCT215600.029.0804148
TGTGCTC179500.027.810819
ACCCGAC31400.027.232783
CTCGGGA106000.026.533582
TTGATCG53950.026.2357522-23
TGATCGC55150.025.32059124-25
GATCGCC58100.024.3210824-25
CCGACTC35250.023.9849575
TCGGGAG118850.023.9029313
TTCGGCT59350.023.77069940-41
ATTCGGC59150.023.64855238-39
TCGCCAG60100.023.35445826-27
Produced by FastQC (version 0.11.5)