FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16189728
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC56900.048.5947468
TCCGACC59050.046.9058769
TTTCCGA71450.039.0979237
GTTTCCG62100.038.555056
CGGGGAT96800.037.8405658
CCGTTTC73650.037.161574
GTCCCGT20850.036.242541
TCGGGGA102950.035.903097
GTTCCGT21000.034.85211
CGTTTCC69600.034.611275
GCTCCGT51400.034.303491
TCCGTTT75600.034.261283
TGTCGGG22800.033.968135
CCCCGTT39950.033.3089332
CCCGTTA18450.032.9717863
CTCCGTT60400.032.5748832
GCCCCGT33500.031.7783071
TCACCCC191850.031.4252289
ATAGCGC101500.031.19043594-95
CCCCCGT31300.030.8233091
Produced by FastQC (version 0.11.5)