FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14931159
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG179020.11989692159865152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC58000.047.4255759
TTCCGAC60400.045.8557368
CGGGGAT123050.039.920968
TGTCGGG28850.038.0413445
TCGGGGA130500.037.751167
TTTCCGA77400.036.3364757
GTTTCCG70550.035.014746
CCCCCGT31000.034.6460951
CCCCGTT43700.034.364522
CCGTTAA17050.033.441174
CCGTTTC80900.032.420164
CTCCGTT64250.032.249132
ATCGGGG90300.032.143866
GTTCCGT22200.031.2541281
CTGTCGG34250.031.2137934
CCCGTTT56450.030.8124453
CGTTTCC79750.030.6807695
ATAGCGC114100.030.47255994-95
TCACCCC205800.030.4481899
GTTCACC206300.030.420457
Produced by FastQC (version 0.11.5)