FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14434360
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGC161800.049.560426
CGGTGTG55150.049.3600655
GTGCTCT220050.048.1000339
GTGTGCT226200.047.06537
TGTGCTC226350.046.740288
TCCGACC45550.041.5093779
CCGGTGT38450.041.3957444
TTCCGAC46950.039.968068
GTCCCGT19700.037.3902631
CCGATCT158050.033.8989616-17
GTTCCGT20200.033.876941
ATTGCGC46850.033.360919
TCCGATC156100.033.303116-17
CGGGGAT65000.032.8898
TTTCCGA59000.032.2076877
TTCCGAT168900.032.0468314-15
CCCCGAA32950.031.585062
CATTGCG49950.031.2904628
CTTCCGA172200.030.64652814-15
CCCCGTT37200.030.531492
Produced by FastQC (version 0.11.5)