FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8304717
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2286442.7531823179525565No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1423921.7145918397941797No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1015241.2224859679143794No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT843811.016061113220354No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG642780.7739938639691154No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG446740.5379352481246501No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG392680.47283971265968483No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC337800.40675678653468866No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA322720.38859843146972983No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT299100.36015676392103424No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA266330.3206972615683352No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC262470.31604930065648235No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC241540.2908467561266687No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA186340.22437850681727023No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT175490.2113136425961294No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC156990.18903714599787083No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG154100.1855571959887375No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG151000.1818243776398401No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG150390.1810898553195732No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC141460.17033693020484625No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC139450.1679166189528192No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG124640.1500833803246998No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA108900.13113029619191116No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG106240.12792729722156695No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC104990.12642212853249546No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG103500.12462796745512218No Hit
TGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCG103050.12408610672705643No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC100370.1208590250576871No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG97820.11778848093198119No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT92840.11179188887472025No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA91210.10982914890417096No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC89880.10822764941899887No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC88230.10624082674942445No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC86590.1042660454293626No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC83350.10036464818728923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA13650.061.6040344
ACCCGAC385600.060.394073
AACCCGA387050.060.2300342
CCGACTC388650.059.8359955
CGACTCC389600.059.6775366
GACTCCC392450.059.2790457
GCGACAT14300.059.1360745
CCCGACT395450.058.891184
ACTCCCT396450.058.582268
CTCCCTT410100.056.655549
CTGCGAC17600.048.5867543
GGGCTCC301000.047.428996
GCTCCCG304250.047.0907148
GTAGCAA109000.046.7635779
GGCTCCC308150.046.4044657
TATTCGT75150.046.2306022
GTATTCG75650.045.8419271
TAGAGCC228500.044.6584367
CCCGGGG322800.044.626582
TAGTAGC113900.044.5037047
Produced by FastQC (version 0.11.5)