FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19090443
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC469740.24606029310058442No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC326350.1709494117030181No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT52400.064.265765
TGCGACA53250.063.423474
CTGCGAC57000.059.3443073
GCTGCGA64250.052.9469762
CGACATC64350.052.479446
GGCTGCG84650.040.9800261
CTAGGCG152300.032.02847
TAGGCGG155100.031.4813168
GCTAGGC180200.027.4387386
TTGATCG63150.027.07814422-23
TGATCGC65200.026.15404524-25
TCGCCAG67500.025.1597226-27
GGCTAGG206950.024.7410455
ATTCGGC68900.024.57732438-39
GATCGCC70450.024.1713124-25
TGGCTAG216200.023.6404974
TGATTCG72200.023.55285536-37
TTCGGCT72650.023.50547240-41
GGCTGGC229300.022.4233021
TCGGCTG77600.021.91426840-41
Produced by FastQC (version 0.11.5)