FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16035422
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG387480.2416400391583084No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA257040.16029512662653966No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG209260.13049859242868694No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG199350.12431852432695566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG43450.050.2980845
TCCGACC97350.049.6296589
TTCCGAC100750.047.8605088
CTGTCGG47250.044.144984
CGGGGAT265750.042.099218
TCGGGGA267250.041.9695787
GTCGGGG54850.041.4865266
TTTCCGA123650.039.1504177
GTTTCCG106550.037.7194256
TCACCCC330350.037.418759
TCTGTCG55850.037.2705463
GTTCCGT28400.036.310921
ATCGGGG198350.035.925866
CGTTTCC113150.035.438175
CCGTTTC117750.034.621434
GTTCACC370900.033.4558947
TTCACCC374950.033.1198658
TCTATCG149550.032.6361433
TATCGGG169350.032.514845
CTATCGG157600.032.409394
Produced by FastQC (version 0.11.5)