FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14934764
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC326520.2186308400989798No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC178310.11939257962161304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA34050.053.5703774
GCGACAT35200.052.3550955
CTGCGAC36850.050.0252383
CGACATC44400.041.9352046
GCTGCGA44200.041.6010172
GGCTGCG55600.034.1018071
CTAGGCG109450.030.7246787
TAGGCGG112150.029.9008858
GCTAGGC135100.025.2080636
GGCTAGG151550.023.223745
ACCCGAC19000.023.0053673
TGGCTAG157600.022.4548764
TTGATCG41750.022.29997422-23
TCGATCG9700.021.54503416-17
CCGATAG56900.021.37012390-91
GCGCGGT26200.021.0296676
TGATCGC44050.020.97389824-25
CGGTCGA83300.020.61320568-69
GGCTGGC170000.020.5735021
AGGCGGG164350.020.5195229
Produced by FastQC (version 0.11.5)