FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17243260
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA381850.2214488443600572No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG271580.157499219985084No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG175360.10169770681414071No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA320850.042.7610249
GCCCCGT42200.042.5667841
TTCGACG5450.040.981971
TTCCGAC51600.040.969748
AGTAGCA352950.038.8989268
TAGTAGC357150.038.228667
CGGGGAT128750.037.5624128
TGTCGGG30000.036.5818025
TCGGGGA136800.035.352057
GTCCCGT22900.035.278081
CCCGTTA19900.035.1045463
CGACCTA11200.034.7926371
GTACGGT16200.034.6143342
CCGTTTC68400.034.3159144
TCAGTCG265350.034.18921784-85
TTTCCGA61750.034.1585127
GTTTCCG55000.034.1176036
AGTCGGT268050.033.6496786-87
TCCGACC62500.033.596599
GTAGTAG260900.033.5033236
Produced by FastQC (version 0.11.5)