FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8871324
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT638350.7195656476981339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299260.33733408902662104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287840.32446115145833926No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93840.10577902464164311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107050.082.518921
GTATCAA306850.070.806481
ATCAACG399500.054.5778733
CAACGCA406750.053.633735
TCAACGC407350.053.5991744
AACGCAG426650.051.111666
TATCAAC445050.049.1802942
ACGCAGA461000.047.275827
CGCAGAG464850.046.8714688
GCAGAGT513350.042.547489
TCTGTCG28950.037.8157128
AGAGTAC533450.031.45831110-11
GTCTCGC25950.030.508481
CTGTCGC37100.030.310359
CTCGCTC25650.028.7760243
CAGAGTA537250.028.68311910-11
CGCTCTG26500.026.9542315
AGTACTT442650.026.3517512-13
TCTCGCT28750.026.0878932
AGTACGG202050.025.95771812-13
Produced by FastQC (version 0.11.5)