FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16401509
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC711030.4335149893829891No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC415060.2530620810560784No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC400770.2443494680885765No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT308330.18798880029880177No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT237220.14463303346051878No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG185500.11309934957813943No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT183980.11217260558159618No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA164450.10026516462601094No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA64000.064.199464
GCGACAT65000.063.719245
CTGCGAC70200.058.674953
CGACATC75000.055.1603476
GCTGCGA75500.054.8087462
GGCTGCG99400.042.1639861
CTAGGCG245450.030.864827
TGGCTAG254550.030.5658194
TAGGCGG253150.029.9077078
ACTCGGG200250.029.0685251
GCTAGGC264550.028.8341986
GGCTAGG279250.027.7243825
CTCGGGA210400.027.6836552
GGCTGGC281800.027.2495821
CTGGCTA292000.026.601473
TCGGGAG219650.026.5173443
TTGATCG79700.025.98567622-23
GACATCT163350.025.907457
GCTGGCT298000.025.7634352
TGATCGC83650.024.70198224-25
Produced by FastQC (version 0.11.5)