FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16401509
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG304000.18534879930864898No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG246520.1503032434393689No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC104450.054.352019
TTCCGAC107200.052.8247688
TGTCGGG39450.047.9291955
TTTCCGA129700.044.0271577
CTGTCGG43550.042.876584
CGGGGAT197500.042.8403478
TCGGGGA203300.041.5947767
GTCGGGG47650.041.3744356
GTTTCCG117550.041.3023876
GTTCCGT28550.041.11391
CGTTTCC117500.039.7447475
CCGTTTC116600.039.282064
TCCGTTT117650.037.4857183
TCACCCC354800.037.4377489
CTCCGTT92400.036.5159762
GTTCACC365650.036.170947
GCTCCGT81250.035.1519171
TTCACCC377550.035.043458
TCTGTCG53350.034.829893
ATCGGGG146700.033.840216
Produced by FastQC (version 0.11.5)