FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16077271
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC599050.3726067688975324No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC467230.2906152418529239No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC275770.17152786688735916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA70500.062.12174
GCGACAT72050.060.846295
CTGCGAC82150.054.0160983
GCTGCGA89750.049.496512
CGACATC90050.048.6314966
GGCTGCG118350.038.1450231
TCGACCG15500.030.0385023
CCGCTCG15900.029.2748957
GACATCT161350.027.6121527
CTAGGCG230100.027.0408957
TAGGCGG239000.026.0542938
ACATCTG172750.025.9004528
TGGCTAG250900.025.4448624
GCTAGGC248000.025.20436
GGCTAGG258100.024.733055
TTGATCG93850.023.56033722-23
TGATCGC96300.022.83783124-25
ACTCGGG98300.022.5276011
CTCGACC20900.022.278052
CTGGCTA294150.021.9176963
Produced by FastQC (version 0.11.5)